A Novel Fluorescent Aptasensor for Sensitive Detection of Oxytetracycline Based on Gold Nanoparticles and OTC-Eu3+ Complex Using Two Different Methods for Modification of Gold Nanoparticles

Hamideh Sharifi Noghabi; Khalil Abnous; Seyed Mohammad Taghdisi; Mahmoud Chamsaz

doi:10.1071/CH21150

RESEARCH ARTICLE

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A Novel Fluorescent Aptasensor for Sensitive Detection of Oxytetracycline Based on Gold Nanoparticles and OTC-Eu³⁺ Complex Using Two Different Methods for Modification of Gold Nanoparticles

Hamideh Sharifi Noghabi ^A , Khalil Abnous ^B ^C ^F , Seyed Mohammad Taghdisi

^D ^E ^F and Mahmoud Chamsaz ^A ^F

+ Author Affiliations

- Author Affiliations

^A Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran.

^B Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad 9177948954, Iran.

^C Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 9177948954, Iran.

^D Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad 9177948954, Iran.

^E Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 9177948954, Iran.

^F Corresponding authors. Email: abnouskh@mums.ac.ir; taghdisihm@mums.ac.ir; mchamsaz@gmail.com

Australian Journal of Chemistry 74(9) 669-675 https://doi.org/10.1071/CH21150
Submitted: 2 July 2021 Accepted: 17 August 2021 Published: 21 September 2021

Abstract

We developed a simple and selective fluorescent aptasensor for the detection of oxytetracycline (OTC) using aptamer-conjugated gold nanoparticles (AuNPs) and a complex formed between oxytetracycline and a europium cation. In this study, AuNPs were modified with an OTC aptamer using two methods (Tween and NaCl methods). In the absence of OTC, an OTC-Eu³⁺ complex binds to the aptamer in the aptamer-conjugated AuNPs to give weak fluorescence emission. However, in the presence of OTC, the aptamer interacts with its target, causing a strong fluorescence emission. Under optimum conditions, the designed method showed high selectivity for OTC and a good linear range to OTC concentration from 15 to 500 nM with a limit of detection (LOD) of 10.6 nM for the NaCl method and linear range over 15–500 nM with an LOD of 8.8 nM for the Tween method. This biosensor was successfully employed to quantify OTC in milk and tablet samples.

Keywords: gold nanoparticles, oxytetracycline, NaCl, fluorescent aptasensor, Tween.

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A Novel Fluorescent Aptasensor for Sensitive Detection of Oxytetracycline Based on Gold Nanoparticles and OTC-Eu3+ Complex Using Two Different Methods for Modification of Gold Nanoparticles

Abstract

References

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A Novel Fluorescent Aptasensor for Sensitive Detection of Oxytetracycline Based on Gold Nanoparticles and OTC-Eu³⁺ Complex Using Two Different Methods for Modification of Gold Nanoparticles