Fluorescent Ion Efflux Screening Assay for Determining Membrane-Active Peptides
Neil M. O’Brien-Simpson A B C F , Wenyi Li D E F , Namfon Pantarat A B C , Mohammed Akhter Hossain D E , Frances Separovic D , John D. Wade D E G and Eric C. Reynolds A B C GA Melbourne Dental School, University of Melbourne, Melbourne, Vic. 3010, Australia.
B Oral Health CRC, University of Melbourne, Melbourne, Vic. 3010, Australia.
C Bio21 Institute, University of Melbourne, Melbourne, Vic. 3010, Australia.
D School of Chemistry, University of Melbourne, Melbourne, Vic. 3010, Australia.
E The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Melbourne, Vic. 3010, Australia.
F These authors contributed equally to this article.
G Corresponding authors. Email: john.wade@florey.edu.au; e.reynolds@unimelb.edu.au
Australian Journal of Chemistry 70(2) 220-228 https://doi.org/10.1071/CH16659
Submitted: 24 November 2016 Accepted: 12 December 2016 Published: 23 December 2016
Abstract
A major global health threat is the emergence of antibiotic-resistant microbes. Coupled with a lack of development of modified antibiotics, there is a need to develop new antimicrobial molecules and screening assays for them. In this study, we provide proof of concept that a large unilamellar vesicle (LUV) method used to study chloride ion efflux facilitated by ionophores and surfactant-like molecules that disrupt membrane integrity can be adapted to identify membrane-interactive antimicrobial peptides (AMPs) and to screen relative activity of AMPs. Lucigenin was encapsulated in LUVs in the presence of Cl– ion (NaCl), which quenches fluorescence, and then incubated with AMPs in 100 mM NaNO3 buffer. Upon AMP membrane interaction or disruption, the Cl– ion is exchanged with the NO3– ion, and the resultant lucigenin fluorescence is indicative of relative AMP activity. Seven AMPs were synthesized by solid-phase peptide chemistry and incubated with LUVs of different phospholipid compositions. Each AMP resulted in lucigenin fluorescence, which was dose dependent, and the relative fluorescence correlated with the minimum inhibitory concentration and minimum bactericidal concentration values for the corresponding peptide. Furthermore, using mammalian model phospholipid LUVs, lucigenin-induced fluorescence also correlated with the AMP cytotoxicity half-maximal inhibitory concentration values. The proline-rich AMP, Chex1-Arg20, which is non-lytic but interacts with the bacterial membrane resulted in lucigenin fluorescence of bacterial membrane model LUVs but not of mammalian membrane model LUVs. The fluorescent ion efflux assay developed here should have applicability for most AMPs and could be tailored to target particular bacterial species membrane composition, potentially leading to the identification of novel membrane-interactive AMPs. The rapid high-throughput method also allows for screening of relative AMP activity and toxicity before biological testing.
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