Binder-Free TiO2 Monolith-Packed Pipette Tips for the Enrichment of Phosphorylated Peptides*
Chang Lei A , Liang Zhou B , Chun Xu A , Xiaoran Sun A , Amanda Nouwens A C and Chengzhong Yu A D
+ Author Affiliations
- Author Affiliations
A Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Qld 4072, Australia.
B State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.
C School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Qld 4072, Australia.
D Corresponding author. Email: c.yu@uq.edu.au
Australian Journal of Chemistry 69(12) 1396-1401 https://doi.org/10.1071/CH16443
Submitted: 29 July 2016 Accepted: 26 August 2016 Published: 21 September 2016
Abstract
A macroporous TiO2 monolith-entrapped pipette-tip was developed through a binder-free packing method for convenient phosphorylated peptide enrichment. A detection limit of 1 ng mL–1 for phosphorylated peptide is achieved, showing a better enrichment efficiency compared with the commercial pure TiO2-embedded NuTip.
References
[1] B. Bodenmiller, L. N. Mueller, M. Mueller, B. Domon, R. Aebersold, Nat. Methods 2007, 4, 231.| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXitFChsbs%3D&md5=bac404df9979539744adaf5187c085d1CAS | 17293869PubMed |
[2] C. J. Marshall, Cell 1995, 80, 179.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXjtlGnsr8%3D&md5=e8b1b6d9f7fad59dee3cc5f6f5f63d9aCAS | 7834738PubMed |
[3] I. Prasadam, T. Friis, W. Shi, S. van Gennip, R. Crawford, Y. Xiao, Bone 2010, 46, 226.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsFynsrrE&md5=bec9d685aa09a8c0db68fdc48cf62ed1CAS | 19853676PubMed |
[4] I. Prasadam, S. van Gennip, T. Friis, W. Shi, R. Crawford, Y. Xiao, Arthritis Rheum. 2010, 62, 1349.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtFGnu7nL&md5=cc3ff3cc0fe0492f8b4c65a222cfbb15CAS | 20155832PubMed |
[5] C. Lei, K. Qian, O. Noonan, A. Nouwensa, C. Z. Yu, Nanoscale 2013, 5, 12033.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhvVejur7O&md5=52060078349502d1a7b26df6afb23356CAS | 24162102PubMed |
[6] L. Qiao, C. Roussel, J. J. Wan, P. Y. Yang, H. H. Girault, B. H. Liu, J. Proteome Res. 2007, 6, 4763.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtlOgsrzK&md5=da296200d14eab4394569fc5acb622d6CAS | 18047269PubMed |
[7] A. Leitner, TrAC, Trends Anal. Chem. 2010, 29, 177.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhslWqsr0%3D&md5=3f8cc4f598f91fe2a187f02e63e7301aCAS |
[8] T. E. Thingholm, T. J. D. Jorgensen, O. N. Jensen, M. R. Larsen, Nat. Protoc. 2006, 1, 1929.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtFagt77N&md5=c0fa84eb2c922f5336e8cd13505ff7c0CAS | 17487178PubMed |
[9] H. Matsuda, H. Nakamura, T. Nakajima, Anal. Sci. 1990, 6, 911.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXnsVCqtg%3D%3D&md5=a217d7cca804789aa5042dc1d853595fCAS |
[10] H. C. Hsieh, C. Sheu, F. K. Shi, D. T. Li, J. Chromatogr. A 2007, 1165, 128.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXpvFKns7o%3D&md5=4273eeb0a23b0669adb57bbdc281222dCAS | 17714720PubMed |
[11] S. Miyazaki, K. Morisato, N. Ishizuka, H. Minakuchi, Y. Shintani, M. Furuno, K. Nakanishi, J. Chromatogr. A 2004, 1043, 19.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlsVSlu7c%3D&md5=18aa7dcf97bfeb058b18909bebc8aa0fCAS | 15317408PubMed |
[12] M. Rainer, H. Sonderegger, R. Bakry, C. W. Huck, S. Morandell, L. A. Huber, D. T. Gjerde, G. K. Bonn, Proteomics 2008, 8, 4593.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVCjtrvK&md5=ccfb2e185133548d974bb21f0152adcaCAS | 18837466PubMed |
[13] R. H. Sui, S. Y. Liu, G. A. Lajoie, P. A. Charpentier, J. Sep. Sci. 2010, 33, 1604.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXmvVGgsL8%3D&md5=ad5ad6eff76af179409e7b103b68bdc4CAS |
[14] T. Kubo, N. Tsujioka, N. Tanaka, K. Hosoya, Mater. Lett. 2010, 64, 177.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsVGntb3N&md5=1730ac5b36857040c76cae93401ba595CAS |
[15] L. Wan, M. C. Long, D. Y. Zhou, L. Y. Zhang, W. M. Cai, Nano-Micro Lett. 2012, 4, 90.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtVenurvK&md5=ef456f52ee1f6402b49d23c73b431d67CAS |
[16] M. Sturm, A. Leitner, J. H. Smatt, M. Linden, W. Lindner, Adv. Funct. Mater. 2008, 18, 2381.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVyltbvM&md5=d12ba8ef951917b1e16912c5d53f88eaCAS |
[17] A. Leitner, M. Sturm, J. H. Smatt, M. Jarn, M. Linden, K. Mechtler, W. Lindner, Anal. Chim. Acta 2009, 638, 51.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXjs1amsLc%3D&md5=b7173039911d1cb91b7982b36a6f1645CAS | 19298879PubMed |
[18] A. Leitner, M. Sturm, O. Hudecz, M. Mazanek, J. H. Smatt, M. Linden, W. Lindner, K. Mechtler, Anal. Chem. 2010, 82, 2726.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXislyqsro%3D&md5=4cf0976672ef8899e9828ff452b097f3CAS | 20201521PubMed |
[19] J. H. Smatt, N. Schuwer, M. Jarn, W. Lindner, M. Linden, Microporous Mesoporous Mater. 2008, 112, 308.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXmtVKgs78%3D&md5=d80ef9ec7f4986a05f436ac550c6383fCAS |
[20] Y. A. Nor, L. Zhou, A. K. Meka, C. Xu, Y. Niu, H. Zhang, N. Mitter, D. Mahony, C. Yu, Adv. Funct. Mater. 2016, 26, 5408.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC28XhtVahtbjI&md5=d50a8a96acc3b529c0d919fd3d1cb161CAS |
[21] M. Wu, Y. Li, Z. Deng, B. L. Su, ChemSusChem 2011, 4, 1481.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtVOmsrbN&md5=25e63e808fe1fb505d16f8887246cb6fCAS | 21994156PubMed |
[22] H. Borchert, E. V. Shevehenko, A. Robert, I. Mekis, A. Kornowski, G. Grubel, H. Weller, Langmuir 2005, 21, 1931.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXosFWisg%3D%3D&md5=84734f203d49dffb5db53d887d5da738CAS | 15723491PubMed |
[23] S. S. Liang, H. Makamba, S. Y. Huang, S. H. Chen, J. Chromatogr. A 2006, 1116, 38.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XksFaltbw%3D&md5=fc0ec1108add0066b055bbdc6743e6aaCAS | 16580007PubMed |
[24] J. Konishi, K. Fujita, K. Nakanishi, K. Hirao, K. Morisato, S. Miyazaki, M. Ohira, J. Chromatogr. A 2009, 1216, 7375.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXht1Gks7bF&md5=418774f0da3d43615ccf21d8ebb035f3CAS | 19580973PubMed |
[25] J. Nawrocki, C. Dunlap, A. McCormick, P. W. Carr, J. Chromatogr. A 2004, 1028, 1.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXltVGnsw%3D%3D&md5=6641c2531b916ab6943778d41c42aeabCAS | 14969280PubMed |
[26] K. Qian, W. Y. Gu, P. Yuan, F. Liu, Y. H. Wang, M. Monteiro, C. Z. Yu, Small 2012, 8, 231.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsFGrsrzO&md5=09ed4cb8aba9dae7a51b999b362643e4CAS | 22135224PubMed |
[27] M. Cuollo, S. Caira, O. Fierro, G. Pinto, G. Picariello, F. Addeo, Rapid Commun. Mass Spectrom. 2010, 24, 1687.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXmt12lsr8%3D&md5=4911446491e9d3f761148b59fc9fc33eCAS | 20486267PubMed |
[28] GlySci, NuTipTM. Available at: http://www.glysci.com/products/NuTip.html (accessed 2 September 2016).
[29] J. M. Zhao, W. X. Niu, L. Zhang, H. R. Cai, M. Y. Han, Y. L. Yuan, S. Majeed, S. Anjum, G. B. Xu, Macromolecules 2013, 46, 140.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhvVGrtbbL&md5=674b18c4e67eac8e9a6fabbcb16b8c40CAS |
