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RESEARCH FRONT
Contents Vol 69(11)

Practical Isolation of Asperuloside from Coprosma quadrifida via Rapid Pressurised Hot Water Extraction

Bianca J. Deans A , Alex C. Bissember A B and Jason A. Smith A B
+ Author Affiliations
- Author Affiliations

A School of Physical Sciences – Chemistry, University of Tasmania, Hobart, Tas. 7001, Australia.

B Corresponding authors. Email: alex.bissember@utas.edu.au; jason.smith@utas.edu.au

Australian Journal of Chemistry 69(11) 1219-1222 https://doi.org/10.1071/CH15743
Submitted: 27 November 2015  Accepted: 1 March 2016   Published: 23 March 2016

Abstract

The valuable glycoside asperuloside was efficiently isolated in multigram quantities from Coprosma quadrifida employing a recently developed rapid pressurised hot water extraction (PHWE) method that utilises an unmodified household espresso machine. This study represents the first time C. quadrifida has been extracted and its major chemical components isolated and analysed. Our findings reveal that this species is a rich source of asperuloside (7.0 % yield w/w). Significantly, this represents one of the highest yields obtained of this glycoside from a Coprosma species, and the entire Rubiaceae family more generally.


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[15]  When we performed the Soxhlet extraction of C. quadrifida (10 g) using acetone for 18 h we were ultimately able to isolate asperuloside in 4.5 % yield w/w. An identical sample of C. quadrifida (10 g) provided asperuloside in 4.9 % yield w/w using our PHWE method. Further details can be found in the Supplementary Material.

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