Label-Free and Sensitive Detection of BRCA1 and TB4 DNA Sequences with Water-Soluble Cationic Polythiophenes

Shaohong Zhou; Huanhuan Ling; Yun Ma; Yan Zhou; Wenqi Du; Meifang Cui; Yong Xia; Liqiang Yan; Hongtao Yao; Zhengjian Qi

doi:10.1071/CH15395

RESEARCH ARTICLE

Label-Free and Sensitive Detection of BRCA1 and TB4 DNA Sequences with Water-Soluble Cationic Polythiophenes

Shaohong Zhou ^A , Huanhuan Ling ^A , Yun Ma ^A , Yan Zhou ^A , Wenqi Du ^A , Meifang Cui ^A , Yong Xia ^A , Liqiang Yan ^A , Hongtao Yao ^A and Zhengjian Qi ^A ^B

+ Author Affiliations

- Author Affiliations

^A College of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu 211189, China.

^B Corresponding author. Email: qizhengjian@seu.edu.cn

Australian Journal of Chemistry 69(4) 473-477 https://doi.org/10.1071/CH15395
Submitted: 1 July 2015 Accepted: 16 September 2015 Published: 1 October 2015

Abstract

A sensitive method for BRCA1 and TB4 DNA sequences detection using water-soluble cationic polythiophenes, poly(3-(1′-ethoxy-2′-N-methyl imidazole)thiophene) (PT) is presented. The fluorescence of PT could be dramatically quenched by the addition of single-stranded DNA (ssDNA; TB4 and BRCA1 sequences). The K_SV (Stern–Volmer constant) for TB4 and BRCA1 DNA sequences are 1.46 × 10⁸ and 3.28 × 10⁸ M^–1 respectively, and the limits of detection of these two sequences are 1.26 × 10^–10 and 0.483 × 10^–10 M respectively. The fluorescence of PT would recover to its initial intensity after the addition of complementary ssDNA, whereas sequences with one to three mismatched bases would not. The influences of buffer pH and concentration of NaCl were also investigated systemically in order to further improve the sensitivity. This assay can be completed in a short period of time without any further procedure. Hence, this sensitive, cost-effective, and rapid detection method for BRCA1 and TB4 DNA sequences may contribute to the clinical diagnosis of breast cancer gene mutations in the future.

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Label-Free and Sensitive Detection of BRCA1 and TB4 DNA Sequences with Water-Soluble Cationic Polythiophenes

Abstract

References

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