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Contents Vol 67(10)

Photoluminescence from Chitosan for Bio-Imaging

Xiaoyong Pan A , Wei Ren A , Liuqun Gu A , Guan Wang A and Ye Liu A B
+ Author Affiliations
- Author Affiliations

A Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602.

B Corresponding author. Email: ye-liu@imre.a-star.edu.sg

Australian Journal of Chemistry 67(10) 1422-1426 https://doi.org/10.1071/CH14274
Submitted: 30 April 2014  Accepted: 2 June 2014   Published: 11 July 2014

Abstract

Photoluminescent behaviours of chitosan were investigated. Photoluminescence can be observed from aqueous solution of chitosan, and CO2 treatment can improve the intensity of photoluminescence. The maximum emission is obtained with an excitation at ~336 nm, and the emission wavelength is dependent on the excitation wavelength with a longer excitation wavelength leading to a longer emission wavelength. The chemistry of chitosan before and after CO2 treatment was characterised; and the results reflect that carbamato anion is formed via the reaction between the amines and CO2, and is the fluorophore of the photoluminescence observed. Furthermore, chitosan was applied as an imaging agent for imaging MCF-7 cells using confocal microscopy. Blue and bright green imaging of the cells can be obtained via tuning the excitation and emission wavelength. Together with a low cytotoxicity reflected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide evaluation, fluorescent chitosan is promising for bio-imaging.


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