Spectroscopic and Modelling Analysis on the Interaction of 3′-Azidodaunorubicin Semicarbazone with ctDNA

Fengling Cui; Jianhua Jin; Xiaoqing Niu; Qingfeng Liu; Guisheng Zhang

doi:10.1071/CH13372

RESEARCH ARTICLE

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Spectroscopic and Modelling Analysis on the Interaction of 3′-Azidodaunorubicin Semicarbazone with ctDNA

Fengling Cui ^A ^B , Jianhua Jin ^A , Xiaoqing Niu ^A , Qingfeng Liu ^A and Guisheng Zhang ^A ^B

+ Author Affiliations

- Author Affiliations

^A College of Chemistry and Chemical Engineering, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang 453007, China.

^B Corresponding authors. Email: fenglingcui@hotmail.com; zgs@htu.cn

Australian Journal of Chemistry 67(2) 234-240 https://doi.org/10.1071/CH13372
Submitted: 16 July 2013 Accepted: 16 September 2013 Published: 17 October 2013

Abstract

The synthesis and characterisation of a new anthracycline, 3′-azidodaunorubicin semicarbazone (ADNRS) is reported. The interaction between ADNRS and calf thymus DNA (ctDNA) was investigated by absorption and fluorescence spectroscopy in combination with melting temperature (T_m) curves and molecular modelling in physiological buffer (pH 7.4). Evidence indicates that ADNRS binds in the groove of ctDNA and the fluorescence quenching mechanism is a static quenching type. Calculated thermodynamic parameters show that hydrophobic interactions may play a predominant role in the binding. Furthermore, molecular modelling results corroborate the spectroscopic investigations.

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