Mass Spectrometry of Gangliosides from Human Sensory and Motor Cortex
Corina Flangea A , Dragana Fabris B , Željka Vukelić B and Alina D. Zamfir A C D
+ Author Affiliations
- Author Affiliations
A Department of Chemical and Biological Sciences, ‘Aurel Vlaicu’ University of Arad, Revolutiei Blvd. 77, 310130, Arad, Romania.
B Department of Chemistry and Biochemistry, University of Zagreb Medical School, Šalata 3, 10000 Zagreb, Croatia.
C Mass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Plautius Andronescu Str. 1, 300224, Timisoara, Romania.
D Corresponding author. Email: alina.zamfir@uav.ro
Australian Journal of Chemistry 66(7) 781-790 https://doi.org/10.1071/CH13173
Submitted: 23 May 2013 Accepted: 10 April 2013 Published: 10 July 2013
Abstract
Sialylated glycosphingolipids, known as gangliosides, are highly expressed in the central nervous system exhibiting region-specific composition in correlation to the specialised functions of particular brain regions. In the present study high resolution tandem mass spectrometry on a quadrupole time-of-flight instrument with nanoelectrospray was optimised and applied for the first comparative assessment of the ganglioside profile in single specimens of adult human motor and somatosensory cortex. In the second stage, the structural analysis performed by collision induced dissociation tandem MS disclosed the presence in motor cortex of a fucose-ganglioside Fuc-GM1 (d18 : 1/20 : 0) isomer exhibiting both N-acetylneuraminic acid and fucose residues linked to the inner galactose.
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