Demonstrating the Use of Bisphenol A-functionalised Gold Nanoparticles in Immunoassays
Joshua R. Peterson A , Yang Lu B , Erwann Luais A C , Nanju Alice Lee B E and J. Justin Gooding A D E
+ Author Affiliations
- Author Affiliations
A School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia.
B School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
C Current address: GREMAN, University François Rabelais, Parc de Grandmont, 37200 Tours, France.
D Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia.
E Corresponding authors. Email: justin.gooding@unsw.edu.au, alice.lee@unsw.edu.au
Australian Journal of Chemistry 66(6) 613-618 https://doi.org/10.1071/CH13043
Submitted: 25 January 2013 Accepted: 5 March 2013 Published: 8 April 2013
Abstract
Spherical gold nanoparticles (5-nm diameter) were modified with a small-molecule thiolated bisphenol A (BPA) ligand to achieve an estimated coverage of ~3.3 × 10–10 mol cm–2, or 180 ligands per particle. The modified particles were tested in an enzyme-linked immunosorbent assay (ELISA) format to measure functionality and were shown to bind specifically to anti-BPA antibody while resisting the non-specific adsorption of an antibody with no affinity for BPA. It was found that the use of 10 % ethanol as a co-solvent was required in the ELISA as aqueous buffers alone resulted in poor binding between anti-BPA antibody and the functionalised nanoparticles. This is likely due to the hydrophobic nature of the BPA ligand limiting its solubility, and therefore its availability for antibody interactions, in purely aqueous environments. To our knowledge, this is the first example of a nanoparticle modified with a small organic molecule being used in an ELISA assay.
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