Uptake and Distribution of a Platinum(ii)-Carborane Complex Within a Tumour Cell Using Synchrotron XRF Imaging
Ellen L. Crossley A , Jade B. Aitken A , Stefan Vogt B , Hugh H. Harris C and Louis M. Rendina A D
+ Author Affiliations
- Author Affiliations
A School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
B X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.
C School of Chemistry and Physics, The University of Adelaide, SA 5005, Australia.
D Corresponding author. Email: lou.rendina@sydney.edu.au
Australian Journal of Chemistry 64(3) 253-257 https://doi.org/10.1071/CH10453
Submitted: 10 December 2010 Accepted: 14 February 2011 Published: 11 March 2011
Abstract
Treatment of A549 human lung carcinoma cells with a DNA metallointercalator complex containing a PtII-terpy (terpy = 2,2′:6′,2′′-terpyridine) unit linked to a functionalized closo-carborane cage results in the uptake of the complex within the cells, as determined by synchrotron X-ray fluorescence (XRF) imaging. Although a significant cellular uptake of Pt existed, there was no significant accumulation of the element within the cell nuclei. Other statistically significant changes from the XRF data included an increase in Cl, K, and Cu and a decrease in Fe within the treated cells.
References
[1] R. F. Barth, J. A. Coderre, M. G. H. Vicente, T. E. Blue, Clin. Cancer Res. 2005, 11, 3987.| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXks1Gksr4%3D&md5=d8a81cdd7d8e38d5416ba0876aaa8adeCAS | 15930333PubMed |
[2] E. L. Crossley, E. J. Ziolkowski, J. A. Coderre, L. M. Rendina, Mini Rev. Med. Chem. 2007, 7, 303.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXitVOitLw%3D&md5=a4fbccd16ea77788e5ac485f2a81b63dCAS | 17346220PubMed |
[3] A. V. Klein, T. W. Hambley, Chem. Rev. 2009, 109, 4911.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVGktbrK&md5=b6baacb9012bd5540685b0cee64b20b9CAS | 19711978PubMed |
[4] J. Gao, Y.-G. Liu, R. A. Zingaro, Chem. Res. Toxicol. 2009, 22, 1705.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVWqtrjN&md5=d6223474b1569e0498ef783f5453cf20CAS | 19694485PubMed |
[5] A. M. Krause-Heuer, R. Gruenert, S. Kuehne, M. Buczkowska, N. J. Wheate, D. D. Le Pevelen, L. R. Boag, D. M. Fisher, J. Kasparkova, J. Malina, P. J. Bednarski, V. Brabec, J. R. Aldrich-Wright, J. Med. Chem. 2009, 52, 5474.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXpsFarsL8%3D&md5=e6d4a8bbd9305db053a8ac50dc54d719CAS | 19658404PubMed |
[6] S. Ahmad, A. A. Isab, S. Ali, Transition Met. Chem. 2006, 31, 1003.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1Wns7vK&md5=087638efc4785cc9128a52943636b2a1CAS |
[7] M. Crul, R. C. A. M. van Waardenburg, J. H. Beijnen, J. H. M. Schellens, Cancer Treat. Rev. 2002, 28, 291.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XpsFyhu7Y%3D&md5=981c36dc3c1072e5673fbf35bf2c9d2fCAS | 12470980PubMed |
[8] A. Ghezzi, M. Aceto, C. Cassino, E. Gabano, D. Osella, J. Inorg. Biochem. 2004, 98, 73.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXpsVSqtro%3D&md5=4b7b50d10106ed58e8b151d3cbdde70cCAS | 14659635PubMed |
[9] E. Wong, C. M. Giandomenico, Chem. Rev. 1999, 99, 2451.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXltFSrsrc%3D&md5=506e9eb65532b283e8861687fd76fd2cCAS | 11749486PubMed |
[10] M. D. Hall, C. T. Dillon, M. Zhang, P. Beale, Z. Cai, B. Lai, A. P. J. Stampfl, T. W. Hambley, J. Biol. Inorg. Chem. 2003, 8, 726.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXnt1KisLc%3D&md5=33a8b77d6d7d6e287536e46df534a2c3CAS | 12884089PubMed |
[11] C. T. Dillon, P. A. Lay, B. J. Kennedy, A. P. J. Stampfl, Z. Cai, P. Ilinski, W. Rodrigues, D. G. Legnini, B. Lai, J. Maser, J. Biol. Inorg. Chem. 2002, 7, 640.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlsVClt7s%3D&md5=51c5b300dd6782b37ee20a572c9ba116CAS | 12072970PubMed |
[12] H. H. Harris, A. Levina, C. T. Dillon, I. Mulyani, B. Lai, Z. Cai, P. A. Lay, J. Biol. Inorg. Chem. 2005, 10, 105.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjtVWmt7c%3D&md5=d5d2533b929d34500b07f77a38dbd422CAS | 15714299PubMed |
[13] H. H. Harris, S. Vogt, H. Eastgate, P. A. Lay, J. Biol. Inorg. Chem. 2008, 13, 303.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtV2ntbk%3D&md5=7761519f078e705531f3435172e9eca8CAS | 18034269PubMed |
[14] M. Ralle, S. Lutsenko, Biometals 2009, 22, 197.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXht1Gntb0%3D&md5=41b4706439ed6841b3ba4401c414d154CAS | 19130257PubMed |
[15] B. S. Twining, S. B. Baines, N. S. Fisher, J. Maser, S. Vogt, C. Jacobsen, A. Tovar-Sanchez, S. A. Sanudo-Wilhelmy, Anal. Chem. 2003, 75, 3806.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXkvFWmtbg%3D&md5=042f8bcb1b72836b647b197454d8034fCAS | 14572047PubMed |
[16] T. Paunesku, S. Vogt, J. Maser, B. Lai, G. Woloschak, J. Cell. Biochem. 2006, 99, 1489.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1Gks7nN&md5=bb7922fb886ff489801e982d1ca3d141CAS | 17006954PubMed |
[17] C. J. Fahrni, Curr. Opin. Chem. Biol. 2007, 11, 121.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXjvFegtb0%3D&md5=c47c73e13f553d4b7debd5948f73b176CAS | 17353139PubMed |
[18] M. D. Hall, R. A. Alderden, M. Zhang, P. J. Beale, Z. Cai, B. Lai, A. P. J. Stampfl, T. W. Hambley, J. Struct. Biol. 2006, 155, 38.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XlvF2nsb8%3D&md5=53079a3ca8710a845462244424f0996fCAS | 16630726PubMed |
[19] K. G. Chen, J. C. Valencia, B. Lai, G. Zhang, J. K. Paterson, F. Rouzaud, W. Berens, S. M. Wincovitch, S. H. Garfield, R. D. Leapman, V. J. Hearing, M. M. Gottesman, Proc. Natl. Acad. Sci. USA 2006, 103, 9903.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XmvVajtLg%3D&md5=b4184150ffe04a3b297794f86009c5f1CAS |
[20] M. Shimura, A. Saito, S. Matsuyama, T. Sakuma, Y. Terui, K. Ueno, H. Yumoto, K. Yamauchi, K. Yamamura, H. Mimura, Y. Sano, M. Yabashi, K. Tamasaku, K. Nishio, Y. Nishino, K. Endo, K. Hatake, Y. Mori, Y. Ishizaka, T. Ishikawa, Cancer Res. 2005, 65, 4998.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXltFemtLw%3D&md5=be84979e999d6a73fb96a65952079527CAS | 15958539PubMed |
[21] J. B. Waern, H. H. Harris, B. Lai, Z. Cai, M. M. Harding, C. T. Dillon, J. Biol. Inorg. Chem. 2005, 10, 443.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtVClt7%2FL&md5=daef9f2afb9f7e5980d3350df82ed316CAS | 15906108PubMed |
[22] L. Yang, R. McRae, M. M. Henary, R. Patel, B. Lai, S. Vogt, C. J. Fahrni, Proc. Natl. Acad. Sci. USA 2005, 102, 11179.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXoslKisb0%3D&md5=27b06b1254e69fe40366fd4ecc47247bCAS |
[23] S. Corezzi, L. Urbanelli, P. Cloetens, C. Emiliani, L. Helfen, S. Bohic, F. Elisei, D. Fioretto, Anal. Biochem. 2009, 388, 33.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXktFamtrY%3D&md5=23750ac6cdb031717bd8cba0de8fe8cfCAS | 19454226PubMed |
[24] J. A. Todd, P. Turner, E. J. Ziolkowski, L. M. Rendina, Inorg. Chem. 2005, 44, 6401.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXntVOksbo%3D&md5=41f4fdf282b5619f1f77764b5c0a98f5CAS | 16124820PubMed |
[25] J. A. Todd, L. M. Rendina, Inorg. Chem. 2002, 41, 3331.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XktVaiu74%3D&md5=946eca22c5e6c77ca90aad0c8513d6bdCAS | 12079447PubMed |
[26] E. L. Crossley, D. Caiazza, L. M. Rendina, Dalton Trans. 2005, 2825.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXntlSisLY%3D&md5=d37efb8136eda070188647c18f52ac96CAS | 16094468PubMed |
[27] F. J. Meyer-Almes, D. Porschke, Biochemistry 1993, 32, 4246.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXitVCrs7c%3D&md5=7ce6ffd546007428e403e0899fae0246CAS | 8476852PubMed |
[28] J. M. Benevides, G. J. Thomas, Biochemistry 2005, 44, 2993.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXps1Kkuw%3D%3D&md5=772ef6e03d8f10b656b202e55d6799d6CAS | 15723542PubMed |
[29] K. W. Jennette, S. J. Lippard, G. A. Vassiliades, W. R. Bauer, Proc. Natl. Acad. Sci. USA 1974, 71, 3839.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2MXpsVeksQ%3D%3D&md5=6841972ad0717cf10747d1db97423dfeCAS |
[30] E. L. Crossley, J. B. Aitken, S. Vogt, H. H. Harris, L. M. Rendina, Angew. Chem. Int. Ed. 2010, 49, 1231.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhslWluro%3D&md5=1a464e561891926e02db12240f41f5b7CAS |
[31] C. D. Ferris, S. R. Jaffrey, A. Sawa, M. Takahashi, S. D. Brady, R. K. Barrow, S. A. Tysoe, H. Wolosker, D. E. Baranano, S. Dore, K. D. Poss, S. H. Snyder, Nat. Cell Biol. 1999, 1, 152.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXks1Ogsb0%3D&md5=925bc67552eb43503ffab7bdd3439da2CAS | 10559901PubMed |
[32] A. Calzolari, V. Finisguerra, I. Oliviero, S. Deaglio, G. Mariani, F. Malavasi, U. Testa, Blood Cells Mol. Dis. 2009, 42, 5.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsFCgsL3P&md5=79df266b799c0cfb710a355840a3c6b6CAS | 19019709PubMed |
[33] H. Chen, M. Costa, Exp. Biol. Med. 2006, 231, 1474.
| 1:CAS:528:DC%2BD28XhtVylt77L&md5=ac1f73f0e0095ac7660a4f224583fcb1CAS |
[34] D. E. Morrison, F. Issa, M. Bhadbhade, L. Gröebler, P. K. Witting, M. Kassiou, P. J. Rutledge, L. M. Rendina, J. Biol. Inorg. Chem. 2010, 15, 1305.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXpvVWhu7c%3D&md5=43dbb11de452503a54e36c821e6189f7CAS | 20694490PubMed |
[35] P. Ilinski, B. Lai, Z. Cai, W. Yun, D. Legnini, T. Talarico, M. Cholewa, L. K. Webster, G. B. Deacon, S. Rainone, D. R. Phillips, A. P. J. Stampfl, Cancer Res. 2003, 63, 1776.
| 1:CAS:528:DC%2BD3sXivFynsrY%3D&md5=6456f32ddbd2afcf2def1979ccc06f08CAS | 12702562PubMed |
[36] S. Vogt, J. Phys. IV 2003, 104, 635.
| Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXksVansLk%3D&md5=d834efc3dfea80d965332c84753784afCAS |
[37] P. van Espen, Handbook of X-Ray Spectrometry: Revised and Expanded 2002 (Eds R. van Grieken, A. Markowicz) (Dekker: New York, NY).
