Time-resolved FT-IR Spectroscopy of Membrane Proteins
Ionela Radu A B , Michael Schleeger A , Melanie Nack A and Joachim Heberle A B
+ Author Affiliations
- Author Affiliations
A Experimental Molecular Biophysics, Department of Physics, Free University of Berlin, Arnimallee 14, D-14195 Berlin, Germany.
B Corresponding authors. Email: iradu@zedat.fu-berlin.de, jheberle@zedat.fu-berlin.de
Australian Journal of Chemistry 64(1) 9-15 https://doi.org/10.1071/CH10286
Submitted: 31 July 2010 Accepted: 9 November 2010 Published: 14 January 2011
Abstract
Time-resolved Fourier transform infrared spectroscopy (FT-IR) offers distinct advantages concerning restrictions pertinent to biomolecules. In particular, it is possible to monitor the temporal evolution of the reaction mechanism of complex machineries as membrane proteins, where other techniques encounter significant experimental difficulties. Here, we present the classical principles and experimental realizations of time-resolved FT-IR spectroscopy together with recent developments employed in our laboratory. Examples from applications to retinal proteins are reviewed that underline the impact of time-resolved FT-IR spectroscopy on the understanding of protein reactions on the level of single bonds.
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