Surface-Enhanced Raman Scattering Spectroscopy of Resveratrol

Jitraporn Vongsvivut; Evan G. Robertson; Don McNaughton

doi:10.1071/CH08204

RESEARCH ARTICLE

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Surface-Enhanced Raman Scattering Spectroscopy of Resveratrol

Jitraporn Vongsvivut ^A , Evan G. Robertson ^A and Don McNaughton ^A ^B

+ Author Affiliations

- Author Affiliations

^A Centre for Biospectroscopy, School of Chemistry, Monash University, Wellington Road, Clayton, Vic. 3800, Australia.

^B Corresponding author. Email: don.mcnaughton@sci.monash.edu.au

Australian Journal of Chemistry 61(12) 921-929 https://doi.org/10.1071/CH08204
Submitted: 14 May 2008 Accepted: 31 July 2008 Published: 10 December 2008

Abstract

We report here, for the first time, the surface-enhanced Raman scattering (SERS) spectra of resveratrol using KNO₃-aggregated citrate-reduced silver (Ag) colloids. The technique provided a substantial spectral enhancement and therefore good quality spectra of resveratrol at parts per million (ppm) concentrations. The detection limit was found to be <1 μM, equivalent to <0.2 ppm. The SERS profile additionally closely resembled its normal solid-state Raman spectrum with some changes in relative intensity. These intensity changes, together with a precise band assignment aided by density functional theory calculations at the B3LYP/6–31G(d) level, allowed the determination of the structural orientation of the adsorbed resveratrol on the surface of the metal nanoparticles. In particular, the SERS spectra obtained at different resveratrol concentrations exhibited concentration-dependent features, suggesting an influence of surface coverage on the orientation of the adsorbed molecules. At a high concentration, an adoption of close-to-upright orientation of resveratrol adsorbed on the metal surface through the p-OH phenyl ring is favoured. The binding structure is, however, altered at lower surface coverage when the concentration decreases to a tilted orientation with the trans-olefin C=C bond aligning closer to parallel to the surface of the Ag nanoparticles.

Acknowledgements

Resveratrol was provided by Food Science Australia (FSA). The DFT calculations were performed through the computers at the Australian Partnership for Advanced Computing (APAC) National Facility. We also gratefully acknowledge Mr Finlay Shanks for assistance with the instrumentations and Dr Peter Godfrey for carrying out the initial computational predictions.

References

Surface-Enhanced Raman Scattering Spectroscopy of Resveratrol

Abstract

Acknowledgements

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