DNA-Binding and Photocleavage Behaviour of [Ru(bpy)2(MDPZ)]2+

Lifeng Tan; Hui Chao; Junjie Fei; Guojun Su; Sheng Zhang; Yue Xia; Liangnian Ji

doi:10.1071/CH08056

RESEARCH ARTICLE

DNA-Binding and Photocleavage Behaviour of [Ru(bpy)₂(MDPZ)]²⁺

Lifeng Tan ^A ^B ^D , Hui Chao ^C , Junjie Fei ^A ^B , Guojun Su ^A ^B , Sheng Zhang ^A ^B , Yue Xia ^A and Liangnian Ji ^C

+ Author Affiliations

- Author Affiliations

^A College of Chemistry, Xiangtan University, Xiangtan 411105, China.

^B Key Lab of Environment-Friendly Chemistry and Application in Ministry of Education, Xiangtan University, Xiangtan 411105, China.

^C Key Laboratory of Bioinorganic and Synthetic Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China.

^D Corresponding author. Email: lfwyxh@yahoo.com.cn

Australian Journal of Chemistry 61(5) 376-381 https://doi.org/10.1071/CH08056
Submitted: 11 February 2008 Accepted: 23 March 2008 Published: 22 May 2008

Abstract

The new ligand MDPZ (MDPZ = 7,7′-methylenedioxyphenyldipyrido[3,2-a:2′,3′-c]-phenazine and its Ru^II complex [Ru(bpy)₂(MDPZ)]²⁺ (phen = 1,10-phenanthroline) was synthesized and characterized by elemental analysis, mass spectrometry, and ¹H NMR spectroscopy. The DNA-binding properties of [Ru(bpy)₂(MDPZ)]²⁺ were investigated by different spectrophotometric methods and viscosity measurements. The results suggest that the complex binds to CT-DNA through intercalation, and can enantioselectively interact with CT-DNA. Although the [Ru(bpy)₂(MDPZ)]²⁺ complex does not show the characteristics of a molecular ‘light switch’ for DNA, [Ru(phen)₂(MDPZ)]²⁺ may serve as a sensitive luminescent probe for DNA. Also, when irradiated at 365 nm, the complex promotes the photocleavage of pBR 322 DNA.

Acknowledgements

The authors thank the Provincial Natural Science Foundation of Hunan (06JJ50023), the Scientific Research Foundation of Hunan Provincial Education Department (06C828), the Key Project of Chinese Ministry of Education (206104), and the Doctoral Foundation of Xiangtan University (05QDZ11) for their support of this research.

References

DNA-Binding and Photocleavage Behaviour of [Ru(bpy)2(MDPZ)]2+

Abstract

Acknowledgements

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DNA-Binding and Photocleavage Behaviour of [Ru(bpy)₂(MDPZ)]²⁺