Interaction Forces of a Supported DOPC Bilayer in the Presence of the General Anaesthetic Halothane — An Atomic Force Microscopy Study

Leanne G. Shamrakov; Zoya V. Leonenko; Eric Finot; David T. Cramb

doi:10.1071/CH06141

RESEARCH ARTICLE

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Interaction Forces of a Supported DOPC Bilayer in the Presence of the General Anaesthetic Halothane — An Atomic Force Microscopy Study

Leanne G. Shamrakov ^A , Zoya V. Leonenko ^A , Eric Finot ^B and David T. Cramb ^A ^C

+ Author Affiliations

- Author Affiliations

^A Department of Chemistry, University of Calgary, Calgary, AB, T2N 1N4, Canada.

^B Laboratory of Physics, Nanosciences, University of Bourgogne, 21078 Dijon cedex, France.

^C Corresponding author. Email: dcramb@ucalgary.ca

Australian Journal of Chemistry 59(6) 386-390 https://doi.org/10.1071/CH06141
Submitted: 3 May 2006 Accepted: 3 June 2006 Published: 6 July 2006

Abstract

In this study atomic force microscopy (AFM) was used to study the effect of halothane on a supported dioleoylphosphatidylcholine (DOPC) bilayer under conditions of high anaesthetic loading. In a previous study we demonstrated that bilayer restructuring occurs as a result of halothane incorporation. Force measurements using AFM indicate an initial decrease in adhesive forces and compressibility between the bilayer and AFM tip, followed by an increase in adhesion properties as a function of incubation time. This effect is attributed to the location and dynamic redistribution of halothane within the bilayer.

Acknowledgments

The authors thank the Natural Sciences and Engineering Council of Canada (NSERC) for funding. L.G.S. acknowledges the Alberta Heritage Foundation for Medical Research for a scholarship.

References

Interaction Forces of a Supported DOPC Bilayer in the Presence of the General Anaesthetic Halothane — An Atomic Force Microscopy Study

Abstract

Acknowledgments

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