Avidin–Biotin Assembling of Horseradish Peroxidase Multi-Monomolecular Layers on Electrodes

Benoît Limoges; Jean-Michel Savéant; Dounia Yazidi

doi:10.1071/CH06039

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Avidin–Biotin Assembling of Horseradish Peroxidase Multi-Monomolecular Layers on Electrodes

Benoît Limoges ^A ^B , Jean-Michel Savéant ^A ^B and Dounia Yazidi ^A

+ Author Affiliations

- Author Affiliations

^A Laboratoire d’Electrochimie Moléculaire, UMR CNRS 7591, Université de Paris 7 ‘Denis Diderot’, 75251 Paris Cedex 05, France.

^B Corresponding authors. Email: limoges@paris7.jussieu.fr; saveant@paris7.jussieu.fr

Australian Journal of Chemistry 59(4) 257-259 https://doi.org/10.1071/CH06039
Submitted: 6 February 2006 Accepted: 23 March 2006 Published: 1 May 2006

Abstract

The strong avidin–biotin affinity is used to stack up successive monomolecular layers of horseradish peroxidase on carbon electrodes. After a biotinylated immunoglobulin is adsorbed on the electrode surface, alternate deposition of neutravidin and biotinylated HRP allows the assemblage of up to 16 successive active HRP layers. The film build-up is followed by cyclic voltammetry using an osmium complex as soluble mediator and H₂O₂ as substrate. The variation of the resulting catalytic responses with H₂O₂ concentration exhibit characteristics qualitatively consistent with the catalysis-inhibition reaction scheme previously established for monomolecular layers. In most cases the catalytic activity increases steadily with the number of monomolecular layers, leading to a significant increase of the analytical sensitivity of the derivatized electrode.

References

Avidin–Biotin Assembling of Horseradish Peroxidase Multi-Monomolecular Layers on Electrodes

Abstract

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