Arsenic Species Determination in Biological Tissues by HPLC–ICP–MS and HPLC–HG–ICP–MS

Jason Kirby; William Maher; Michael Ellwood; Frank Krikowa

doi:10.1071/CH04094

RESEARCH ARTICLE

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Arsenic Species Determination in Biological Tissues by HPLC–ICP–MS and HPLC–HG–ICP–MS

Jason Kirby ^A ^B ^D , William Maher ^A , Michael Ellwood ^C and Frank Krikowa ^A

+ Author Affiliations

- Author Affiliations

^A EcoChemistry Laboratory, Division of Health, Design and Science, University of Canberra, Bruce ACT 2616, Australia.

^B Current address: CSIRO Land and Water, Glen Osmond SA 5867, Australia.

^C National Institute of Water and Atmospheric Research, PO Box 11 115, Hamilton, New Zealand.

^D Author to whom correspondence should be addressed (e-mail: jason.kirby@csiro.au).

Australian Journal of Chemistry 57(10) 957-966 https://doi.org/10.1071/CH04094
Submitted: 23 April 2004 Accepted: 4 July 2004 Published: 1 October 2004

Abstract

The use of high-pressure liquid chromatography coupled directly or by a hydride generation system to an inductively coupled plasma mass spectrometer for the unambiguous measurement of 13 arsenic species in marine biological extracts is described. The use of two chromatography systems; a Supelcosil LC-SCX cation-exchange column eluted with a 20 mM pyridine mobile phase adjusted to pH 2.2 and 2.6 with formic acid, with a flow rate of 1.5 mL min⁻¹ at 40°C, and a Hamilton PRP-X100 anion-exchange column eluted with 20 mM NH₄H₂PO₄ buffer at pH 5.6, with a flow rate of 1.5 mL min⁻¹ at 40°C, was required to separate and quantify cation and anion arsenic species. Under these conditions, arsenous acid could not be separated from other arsenic species and required the use of an additional hydride generation step. Arsenic species concentrations in a locally available Tasmanian kelp (Durvillea potatorum), a certified reference material (DORM-2), and a range of commercially available macroalgae supplements and sushi seaweeds have been measured and are provided for use as in-house quality control samples to assess the effectiveness of sample preparation, extraction, and measurement techniques.

References

Arsenic Species Determination in Biological Tissues by HPLC–ICP–MS and HPLC–HG–ICP–MS

Abstract

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