New DNA Modification Strategies Involving Oxime Formation
Ben Cebon,
John N. Lambert, Donmienne Leung, Hugh Mackie, Karen L. McCluskey, Xuan Nguyen and Claudia Tassone
Australian Journal of Chemistry
53(4) 333 - 339
Published: 2000
Abstract
A new method for the formation of covalently linked DNA conjugates is described. The method involves installation of a 5´-hydroxylamine nucleophile onto synthetic DNA and condensation with a suitable electrophilic carbonyl compound to form an oxime linkage. Various protection strategies for the hydroxylamine group and their merits are discussed and the formation of an oligonucleotide–peptide conjugate is described.Keywords: Conjugate; DNA; oligonucleotide; oxime; peptide; phosphoramidite; synthesis.
https://doi.org/10.1071/CH00018
© CSIRO 2000
