Amino Acid Sequences Containing Cysteine or Cystine Residues in Ovalbumin From Eggs of the Quail Coturnix Coturnix Japonica

Abstract

Ovalbumin isolated from eggs of the Japanese quail, C. c. japonica, was subjected to limited proteolysis by subtilisin to give plakalbumin and then fractionated on Sephadex G75 in acid-urea to give plakalbumin S-protein and S-peptide. The plakalbumin peptide was recovered, oxidized with performic acid, and the sequence of amino acids determined from the peptides formed by enzyme digestion.