Physical Analysis of a (dG+dC)-Rich Fraction of DNA Obtained From the Ovine Genome

Abstract

Using the organomercuric compound 3,6-bis(acetatomercurimethyl)dioxane in conjunction with CS2S04 density gradient equilibrium centrifugation a (dG+dC)-rich DNA fraction constituting 10% of the ovine genome was separated from the remainder. Further fractionation allowed four distinct classes of DNA to be identified with buoyant densities in neutral CsCI of 1· 714, 1· 717, 1 ·725 and 1·716 g/cm3 ? Computerized curve resolving of the data indicated the presence of several additional DNA classes.