Effect of Dimethyl Sulphoxide on the Expression of Nitrogen Fixation in Bacteria
Mary L Skotnicki and Barry G Rolfe
Australian Journal of Biological Sciences
30(2) 141 - 154
Published: 1977
Abstract
Storage in dimethyl sulphoxide (DMSO) of Escherichia coli K12 hybrids carrying nif+ genes from Klebsiella pneumoniae can result in selection of a defective nitrogen-fixing phenotype. Similar results are obtained with E. coli K12 hybrids containing the nitrogep-fixing capacity from Rhizobium trifolii. DMSO appears to affect particular inner membrane proteins associated with energy metabolism in E. coli K12 and four chromosomal regions (chID, chlG, his and unc) are associated with resistance to DMSO.https://doi.org/10.1071/BI9770141
© CSIRO 1977
