The Amino Acid Sequence of the Large Plakalbumin Peptide and the C-Terminal Sequence of Ovalbumin

Abstract

The amino acid sequence of a 33-residue peptide isolated from plakalbumin by gel filtration in 6M urea at pH 3 and derived from the C-terminal portion of ovalbumin has been determined. Enzyme digestion of the hydrophobic areas by thermolysin, papain, ana subtilisin BPN' gave peptides with overlapping sequences. The peptides were fractionated by a combination of paper ionophoresis and chromatography and their sequences determined by the dansyl-Edman technique.