Ionic and Osmotic Effects on Cell Volume and Oxygen Consumption of Rat Bone Marrow Cells

Abstract

Two problems frequently encountered in metabolic studies are osmotic balance (Troshin 1966) and ion effect (Bacq 1965). The ions used in this study were the common buffering ions bicarbonate and phosphate, and a substituted taurine, N( 2-hydroxyethyl)piperazine-N'-2'-ethanesulphonic acid (HEPES), recently synthesized by Good et al. (1966). It was felt that a comparison of changes in cell volume and oxygen consumption induced by alterations in various media constituents might disclose some of the effects that cell volume and ion content (MacLeod and Rhoads 1939; Pages and Delaunay 1945; Evans and Bird 1949) have on cellular metabolism