Adsorption Titration as a Specific Semi-Quantitative Assay for Soluble and Bound Paramecium Serotypic Antigen
Helen Macindoe and AH Reisner
Australian Journal of Biological Sciences
20(1) 141 - 152
Published: 1967
Abstract
The immobilization of paramecia when mixed with appropriate antisera has been utilized as a specific and semi· quantitative assay for the serotypic antigens (adsorption titration). While serotypic antigen in both soluble and particle·bound forms is detected by adsorption titration, only soluble antigen is detected in gel.diffusion analysis. 500 g, 3000 g, 12,000 g, and 105,000 g particulate fractions as well as 105,000 g and 150,000 g cell supernatant fractions were prepared by differential centrifugation. Analyses of these fractions indicate:https://doi.org/10.1071/BI9670141
© CSIRO 1967
