The Conversion of Gentisic Acid to Maleylpyruvic Acid by an Aohromobaoter Species

Abstract

Simultaneous adaptation experiments with washed cell suspensions of an Achromobacter species indicate that this organism metabolizes anthranilic acid and N-(carboxymethyl)anthranilic acid (CMAA) via gentisic acid (Ladd, unpublished data). It is the purpose of this communication to show that cell-free extracts, prepared from cells adapted to anthranilic acid or CMAA, oxidize gentisic acid to maleylpyruvic acid similarly to Pseudomonas spp. used by Lack (1959, 1961) and by Sugiyama et al. (1960).