Studies of the Growth Substances of Legume Nodules Using Paper Chromatography

Abstract

Five distinct growth substances active in the Avena coleoptile straightgrowth test were separated by paper chromatography of ethanol extracts of nodules of Pisum arvense L. and Ulex europaeus L. Three of the active substances were classed as promotors, two as inhibitors, of coleoptile elongation. They exhibited the following migration behaviour in isopropanol-ammonia chromatography solvent: