A Critical Study of the Use of Paper Electrophoresis for Separating Proteins and Measuring Their Isoelectric Points

Abstract

A study has been made of the factors affecting the migration of proteins during electrophoresis on paper strips held between glass plates. A major difficulty in interpreting results is caused by movement of liquid in the paper from anode to cathode, the rate of flow diminishing from the anode end to the cathode end. Even with non-uniform liquid flow of this type it is possible to choose a path such that migration of an ion is linear with respect to both time and potential gradient.