Register      Login
Australian Journal of Biological Sciences Australian Journal of Biological Sciences Society
Biological Sciences
RESEARCH ARTICLE

Studies on the Nitrate Reductase of Escherichia Coli in the Cell-Free State

W Joklik

Australian Journal of Biological Sciences 3(1) 28 - 44
Published: 1950

Abstract

A method of purifying the nitrate reductase obtained by crushingEscherichia coli cells in a ground glass mill is described, involving elution atpH 9.5 and precipitation into slightly acidified acetone, followed by treatmentwith phosphate buffer ( pH 8.0) and dialysis. Methods of estimating theactivity of the enzyme without recourse to coupling with other enzymes areoutlined, viz.: (a) the oxidation time method; (b) the use of photochemicallyreduced methylene blue as hydrogen donor.

https://doi.org/10.1071/BI9500028

© CSIRO 1950

PDF (4.1 MB) Export Citation

Share

Share on Facebook Share on Twitter Share on LinkedIn Share via Email

View Dimensions