Isolation and characterisation of microsatellite loci in the bush stone-curlew (Burhinus grallarius), a declining Australian bird
Robert A. B. Mason A D E , Catherine Price B , Walter E. Boles A , Karen-Anne Gray A , Edwina Rickard A C , Mark D. B. Eldridge A and Rebecca N. Johnson AA Australian Museum Research Institute, Australian Centre for Wildlife Genomics, Australian Museum, 6 College Street, Sydney, NSW 2010, Australia.
B School of Biological Sciences, The University of Sydney, Sydney, NSW 2006, Australia.
C Department of Clinical Genetics, The Children’s Hospital at Westmead, Westmead, NSW 2145, Australia.
D Present address: School of Biological Sciences, The University of Queensland, St Lucia, Qld 4072, Australia, and Hawai‘i Institute of Marine Biology, University of Hawai‘i at Mānoa, 46-007 Lilipuna Road, Kane‘ohe, HI 96744, USA.
E Corresponding author. Email: robert.mason1@uqconnect.edu.au
Australian Journal of Zoology 61(6) 421-423 https://doi.org/10.1071/ZO13059
Submitted: 25 July 2013 Accepted: 6 January 2014 Published: 28 January 2014
Abstract
The bush stone-curlew (Burhinus grallarius Latham), a ground-nesting nocturnal bird, is endangered in southern Australia due to habitat modification and introduced predators. To provide tools for conservation, ecological and behavioural studies, we isolated variable microsatellite repeat sequences and designed primers for PCR amplification in this species. Primer pairs were developed and levels of diversity were assessed for eight microsatellite loci, including one locus linked to the gene encoding Microtubule-Associated Protein 2, a protein important for behavioural imprinting in birds, and one sex-linked locus. Isolated loci contained allelic diversity of between 5 and 17 alleles.
Additional keywords: Burhinidae, conservation genetics, hemizygous, hybridisation capture and enrichment, microsatellite, Microtubule-Associated Protein 2 (MAP2), non-isotopic screening.
References
Andrews, L. M. (1997). The breeding behavior and success of the bush stone-curlew (Burhinus grallarius) on Magnetic Island. B.Sc.(Honours) Thesis, James Cook University, Townsville.Bickart, K. J. (1981). A new thick-knee, Burhinus, from the Miocene of Nebraska, with comments on the habitat requirements of the Burhinidae (Aves: Charadriiformes). Journal of Vertebrate Paleontology 1, 273–277.
| A new thick-knee, Burhinus, from the Miocene of Nebraska, with comments on the habitat requirements of the Burhinidae (Aves: Charadriiformes).Crossref | GoogleScholarGoogle Scholar |
Carter, A. (2010). Improving red fox (Vulpes vulpes) management for bush stone curlew (Burhinus grallarius) conservation in south-eastern Australia. Ph.D. Thesis, Charles Sturt University, Albury.
Davies, P. A., and Gray, G. (2002). Long-range PCR. In ‘PCR Mutation Detection Protocols: Methods in Molecular Biology. Volume 187’. (Eds B. D. M. Theophilus and R. Rapley.) pp. 51–55. (Humana Press: Totowa.)
DEC NSW (2006). NSW recovery plan for the bush stone-curlew Burhinus grallarius. Department of Environment and Conservation, New South Wales, Sydney.
Excoffier, L., and Lischer, H. E. L. (2010). Arlequin Suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10, 564–567.
| Arlequin Suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows.Crossref | GoogleScholarGoogle Scholar | 21565059PubMed |
Garnett, S. T., and Crowley, G. M. (2000). ‘The Action Plan for Australian Birds 2000.’ (Environment Australia: Canberra.)
Glenn, T. C., and Schable, N. A. (2005). Isolating microsatellite DNA loci. Methods in Enzymology 395, 202–222.
| Isolating microsatellite DNA loci.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XotlGrtb4%3D&md5=9f0ca5d31dbe71d6f040a857f3905207CAS | 15865969PubMed |
Hamilton, M. B. (2009). ‘Population Genetics.’ (Wiley-Blackwell: New Jersey.)
Haqqi, T., Zhao, X., Panciu, A., and Yadav, S. P. (2002). Sequencing in the presence of betaine: improvement in sequencing of the Localized Repeat Sequence Regions. Journal of Biomolecular Techniques 13, 265–271.
| 1:STN:280:DC%2BD1Mzps1Kiuw%3D%3D&md5=2ab12d2778ef6684c4c52d61f2e3d857CAS | 19498992PubMed |
Kahn, N. W., St. John, J., and Quinn, T. W. (1998). Chromosome-specific intron size differences in the avian CHD gene provide an efficient method for sex identification in birds. The Auk 115, 1074–1078.
| Chromosome-specific intron size differences in the avian CHD gene provide an efficient method for sex identification in birds.Crossref | GoogleScholarGoogle Scholar |
Rozen, S., and Skaletsky, H. J. (2000). Primer3 on the WWW for general users and for biologist programmers. In ‘Bioinformatics Methods and Protocols: Methods in Molecular Biology. Volume 132’. (Eds S. Krawetz and S. Misener.) pp. 365–386. (Humana Press: Totowa.)
Van Oosterhout, C., Hutchinson, W. F., Wills, D. P. M., and Shipley, P. (2004). Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4, 535–538.
| Micro-checker: software for identifying and correcting genotyping errors in microsatellite data.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXnvFOktb8%3D&md5=642d3c27a53cc46b25cea2a96ea87beeCAS |
Wahlund, S. (1928). Zusammensetzung von Populationen und Korrelationserscheinungen vom Standpunkt der Vererbungslehre aus Betrachtet. Hereditas 11, 65–106.
| Zusammensetzung von Populationen und Korrelationserscheinungen vom Standpunkt der Vererbungslehre aus Betrachtet.Crossref | GoogleScholarGoogle Scholar |
Wimmer, J., Towsey, M., Planitz, P., Roe, P., and Williamson, I. (2010). Scaling acoustic data analysis through collaboration and automation. In ‘Proceedings of the 2010 Sixth IEEE International Conference on e-Science’. pp. 308–315. (IEEE Computer Society: Washington, DC.)
Yamaguchi, S, Fujii-Taira, I, Murakami, A, Hirose, N, Aoki, N, Izawa, E, Fujimoto, Y, Takano, T, Matsushima, T, and Homma, K. J. (2008). Up-regulation of microtubule-associated protein 2 accompanying the filial imprinting of domestic chicks (Gallus gallus domesticus). Brain Research Bulletin 76, 282–288.
| Up-regulation of microtubule-associated protein 2 accompanying the filial imprinting of domestic chicks (Gallus gallus domesticus).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXmtlSkt70%3D&md5=8818657dfa8a790898b4349a72060bc6CAS | 18498942PubMed |
Yamaguchi, S., Katagiri, S., Aoki, N., Iikubo, E., Kitajima, T., Matsushima, T., and Homma, K. J. (2011). Molecular function of microtubule-associated protein 2 for filial imprinting in domestic chicks (Gallus gallus domesticus). Neuroscience Research 69, 32–40.
| Molecular function of microtubule-associated protein 2 for filial imprinting in domestic chicks (Gallus gallus domesticus).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFajt7zL&md5=78e7284f981eb65cb8fa2136651e9e00CAS | 20858519PubMed |
