Infrageneric phylogeny of Chloantheae (Lamiaceae) based on chloroplast ndhF and nuclear ITS sequence data
B. J. Conn A D , N. Streiber A B , E. A. Brown A , M. J. Henwood B and R. G. Olmstead CA National Herbarium of New South Wales, Mrs Macquaries Road, Sydney, NSW 2000, Australia.
B School of Biological Sciences, University of Sydney, NSW 2006, Australia.
C Department of Biology, University of Washington, Box 355325, Seattle, WA 98195-1330, USA.
D Corresponding author. Email: barry.conn@rbgsyd.nsw.gov.au
Australian Systematic Botany 22(4) 243-256 https://doi.org/10.1071/SB09011
Submitted: 18 March 2009 Accepted: 18 June 2009 Published: 31 August 2009
Abstract
The tribe Chloantheae (Prostantheroideae, Lamiaceae) currently consists of over 100 species in nine genera, all of which are endemic to Australia. Generic delimitations were assessed using chloroplast 3′ndhF and nuclear ITS nucleotide sequence data for up to seventy species. Analyses of the two datasets, independently and in combination, used maximum parsimony and Bayesian phylogenetic inference methods. Topologies derived from each marker were broadly congruent, but better resolution and stronger branch support was achieved by combining the datasets. The monophyly of the Chloantheae was confirmed. Brachysola is sister to the rest of the tribe and Chloanthes, Cyanostegia and Dicrastylis (including Mallophora) are monophyletic. Although the species within Dicrastylis were only partially resolved, it appears likely that the current sectional classification of this genus will require revision. A clade containing Newcastelia, Physopsis and Lachnostachys (=Physopsideae) was recovered, but the topology indicates that the current generic circumscriptions need further investigation. A close relationship between Hemiphora elderi, Pityrodia bartlingii and P. uncinata was resolved and reflects their palynological and carpological similarities. The relationship between remaining species of Pityrodia was incompletely resolved.
Acknowledgements
This research was supported by the Australian Federation of University Women, Australian Geographic, Australian Systematic Botany Society Inc., International Association for Plant Taxonomy, Linnaean Society of New South Wales, Royal Botanic Gardens and Domain Trust Sydney, and University of Sydney grants to NS and US NSF grants DEB-9509804 and DEB-0542493 to RGO. We sincerely thank the many people who provided advice and technical assistance throughout this study; in particular, we thank staff at the University of Sydney and the National Herbarium of New South Wales. Field assistance and plant identification was provided by Bob Coveny (NSW) and Peter Jobson (then NSW). Barbara Rye (PERTH) also generously assisted with plant identifications. Ahmad Abid Munir (AD) provided advice on the systematics of Chloantheae. Adam Marchant, John Thomson and Margaret Heslewood (all NSW) provided assistance with molecular laboratory techniques. Carolyn Porter (NSW) and Patrick Reeves (UW) also provided molecular laboratory assistance and provided additional ITS and ndhF sequence data. We thank Chris Quinn (NSW) for his sound advice on the analysis of molecular data. The directors, curators and staff of AD, K, PERTH and PRH kindly arranged the loan of plant specimens.
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