Implication of transcriptome profiling of spermatozoa for stallion fertility
Yara Suliman A , Frank Becker A C and Klaus Wimmers B
+ Author Affiliations
- Author Affiliations
A Institute for Reproductive Biology, Leibniz Institute for Farm Animal Biology Dummerstorf, D-18196 Dummerstorf, Wilhem-Stahl-Allee 2, Germany.
B Institute for Genome Biology, Leibniz Institute for Farm Animal Biology Dummerstorf, D-18196 Dummerstorf, Wilhelm-Stahl-Allee 2, Germany.
C Corresponding author. Email: becker@fbn-dummerstorf.de
Reproduction, Fertility and Development 30(8) 1087-1098 https://doi.org/10.1071/RD17188
Submitted: 9 July 2016 Accepted: 6 December 2017 Published: 14 March 2018
Journal Compilation © CSIRO 2018 Open Access CC BY-NC-ND
Abstract
Poor fertility of breeding stallions is a recognised problem in the equine industry. The aim of the present study was to detect molecular pathways using two groups of stallions that differed in pregnancy rates as well as in the proportion of normal and motile spermatozoa. RNA was isolated from spermatozoa of each stallion and microarray data were analysed to obtain a list of genes for which transcript abundance differed between the groups (P ≤0.05, fold change ≥1.2). In all, there were 437 differentially expressed (DE) genes between the two groups (P ≤ 0.05, fold change ≥1.2). Next, the DE genes were analysed using Database for Annotation, Visualisation, and Integrated Discovery (DAVID). Finally, ingenuity pathways analysis (IPA) was used to identify top biological functions and significant canonical pathways associated with the DE genes. Analysis using the DAVID database showed significant enrichment in the gene ontology (GO) term ‘RNA binding’ (P = 0.05) and in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cytokine–cytokine receptor interaction (P = 0.02). Furthermore, IPA analysis showed interconnected biological functions and canonical pathways involved in the regulation of spermatogenesis and male fertility. In addition, significantly enriched metabolic pathways were identified. In conclusion, the present study has identified, for the first time, molecular processes in stallion spermatozoa that could be associated with stallion fertility.
Additional keywords: microarray, sperm RNA, spermatogenesis.
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