Microtubule stabilisers docetaxel and paclitaxel reduce spindle damage and maintain the developmental competence of in vitro-mature bovine oocytes during vitrification
Jakkhaphan Pitchayapipatkul A F , Tamás Somfai B , Satoko Matoba B , Rangsan Parnpai C , Takashi Nagai D , Masaya Geshi B and Thevin Vongpralub E
+ Author Affiliations
- Author Affiliations
A Faculty of Agriculture, Princess of Naradhiwas University, Narathiwat 96000, Thailand.
B Animal Breeding and Reproduction Research Division, National Agriculture and Food Research Organisation (NARO) Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.
C Embryo Technology and Stem Cell Research Center, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
D Food and Fertilizer Technology Center, Taipei 10648, Taiwan.
E Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
F Corresponding author. Email: jakkhaphan@gmail.com
Reproduction, Fertility and Development 29(10) 2028-2039 https://doi.org/10.1071/RD16193
Submitted: 7 May 2016 Accepted: 10 December 2016 Published: 2 February 2017
Abstract
This study compared the efficacy of docetaxel (DT) and paclitaxel (PT) in reducing spindle damage during vitrification and maintaining the developmental competence of in vitro-matured (IVM) bovine oocytes after vitrification and warming. Pretreatment of IVM oocytes with 0.05 µM DT for 30 min before vitrification resulted in significantly higher (P < 0.05) rates of oocyte survival and cleavage after IVF, as well as subsequent blastocyst rates on Days 7–9 and hatching on Days 8–9, compared with oocytes pretreated with 1.0 µM PT before vitrification or those vitrified without pretreatment. When nuclear status and spindle morphology of vitrified oocytes were assess after warming by immunostaining, DT pretreatment before vitrification resulted in a significantly higher (P < 0.05) percentage of oocytes at the MII stage with a normal, intact spindle compared with PT pretreatment or no pretreatment, but the percentage of MII oocytes was still significantly lower (P < 0.05) than in the control group. Pretreatment of IVM bovine oocytes with 0.05 µM DT or 1.0 µM PT for 30 min before vitrification reduces spindle damage to the same extent, without side effects on fertilisation and development. Pretreatment with 0.05 µM DT improved the developmental competence of vitrified–warmed oocytes to a greater degree than 1.0 µM PT pretreatment.
Additional keywords: cattle, cryotolerance, cytoskeleton, disassembly, freezing, germplasm, metaphase II, taxanes.
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