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RESEARCH ARTICLE

Influence of globulin subunit composition of soybean proteins on silken tofu quality. 2. Absence of 11SA4 improves the effect of protein content on tofu hardness

Andrew T. James A and Aijun Yang A B
+ Author Affiliations
- Author Affiliations

A CSIRO Plant Industry, 306 Carmody Road, St Lucia, Qld 4067 Australia.

B Corresponding author. Email: Aijun.Yang@csiro.au

Crop and Pasture Science 65(3) 268-273 https://doi.org/10.1071/CP13399
Submitted: 19 November 2013  Accepted: 24 January 2014   Published: 14 April 2014

Abstract

Soybean variety Bunya was developed in Australia to provide a better quality bean for tofu manufacturers. It is null for globulin subunit 11SA4. We investigated the effect of both the Bunya genetic background and the11SA4 subunit on tofu properties using genotypes containing 11SA4, with and without Bunya parentage, or lacking 11SA4 with Bunya parentage. Both Bunya parentage and 11SA4 significantly influenced globulin subunit composition and tofu texture. The group lacking 11SA4 had lower seed protein content, the largest seeds and the highest 7S and the lowest 11S content and produced the hardest tofu. Examination of the impact of 11SA4 null on tofu texture at four protein contents (380–440 g kg–1) over four coagulant (2.0–3.5 g kg–1) levels revealed that the absence of 11SA4 produced firmer tofu across the protein and coagulation levels tested, and this difference was larger than that from higher protein or coagulation levels. These results demonstrated that the absence of the 11SA4 subunit could increase tofu hardness to a level that otherwise could only be achieved with much higher seed protein content.


References

Cai TD, Chang KC (1999) Processing effect on soybean storage proteins and their relationship with tofu quality. Journal of Agricultural and Food Chemistry 47, 720–727.
Processing effect on soybean storage proteins and their relationship with tofu quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXjtFSntA%3D%3D&md5=23963976ad91a3814fe6f78ebf3334aeCAS |

Cai R, McCurdy A, Baik BK (2002) Textural property of 6 legume curds in relation to their protein constituents. Journal of Food Science 67, 1725–1730.
Textural property of 6 legume curds in relation to their protein constituents.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlvV2nsbY%3D&md5=cb7b2d44c2ce7cccefb46d1c002534c7CAS |

Cui Z, James AT, Mizazaki S, Wilson RF, Carter TE (2004) ‘Breeding specialty soybeans for traditional and new soyfoods in soybeans as functional foods and ingredients.’ (Ed. K Liu) pp. 264–322 (AOCS Press: Champaign, IL)

Delannay X, Rodgers DM, Palmer RG (1983) Relative genetic contributions among ancestral lines to north-American soybean cultivars. Crop Science 23, 944–949.
Relative genetic contributions among ancestral lines to north-American soybean cultivars.Crossref | GoogleScholarGoogle Scholar |

Evans DE, Tsukamoto C, Nielsen NC (1997) A small scale method for the production of soymilk and silken tofu. Crop Science 37, 1463–1471.
A small scale method for the production of soymilk and silken tofu.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXmslCmtLY%3D&md5=a7eea0b9d6a49036a0267688fdc05a86CAS |

James AT, Lawn RJ (2011) Application of physiological understanding in soybean improvement. II. Broadening phenological adaptation across regions and sowing dates. Crop & Pasture Science 62, 12–24.
Application of physiological understanding in soybean improvement. II. Broadening phenological adaptation across regions and sowing dates.Crossref | GoogleScholarGoogle Scholar |

Ji MP, Cai TD, Chang KC (1999) Tofu yield and textural properties from three soybean cultivars as affected by ratios of 7S and 11S proteins. Journal of Food Science 64, 763–767.
Tofu yield and textural properties from three soybean cultivars as affected by ratios of 7S and 11S proteins.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXntlGqsLY%3D&md5=82233b739862204fa638e734eb719852CAS |

Khatib KA, Aramouni FM, Herald TJ, Boyer JE (2002) Physicochemical characteristics of soft tofu formulated from selected soybean varieties. Journal of Food Quality 25, 289–303.
Physicochemical characteristics of soft tofu formulated from selected soybean varieties.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XovVWisro%3D&md5=b3d8e259f2420061ae2f43b3b3f00e3eCAS |

Kim Y, Wicker L (2005) Soybean cultivars impact quality and function of soymilk and tofu. Journal of the Science of Food and Agriculture 85, 2514–2518.
Soybean cultivars impact quality and function of soymilk and tofu.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht1Grt77L&md5=0a3d5c6e2cc984d7bd35fe94127435a5CAS |

Mahmoud AA, Natarajan SS, Bennett JO, Mawhinney TP, Wiebold WJ, Krishnan HB (2006) Effect of six decades of selective breeding on soybean protein composition and quality: A biochemical and molecular analysis. Journal of Agricultural and Food Chemistry 54, 3916–3922.
Effect of six decades of selective breeding on soybean protein composition and quality: A biochemical and molecular analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XjvFWksbs%3D&md5=66ef85ef7a3e77c12ecb2f48261bf897CAS | 16719515PubMed |

Min S, Yu Y, St Martin S (2005) Effect of soybean varieties and growing locations on the physical and chemical properties of soymilk and tofu. Journal of Food Science 70, C8–C21.
Effect of soybean varieties and growing locations on the physical and chemical properties of soymilk and tofu.Crossref | GoogleScholarGoogle Scholar |

Mujoo R, Trinh DT, Ng PKW (2003) Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture. Food Chemistry 82, 265–273.
Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXktF2ltr8%3D&md5=e4ac757a56902bc8d002029fb4719f0bCAS |

Murphy PA, Chen HP, Hauck GC, Wilson LA (1997) Soybean protein composition and tofu quality. Food Technology 51, 86–88.

Nakamura T, Utsumi S, Kitamura K, Harada K, Mori T (1984) Cultivar differences in gelling characteristics of soybean glycinin. Journal of Agricultural and Food Chemistry 32, 647–651.
Cultivar differences in gelling characteristics of soybean glycinin.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXitVyju78%3D&md5=6e27a06d70945a0c90e7733de3236285CAS |

Nielsen NC, Dickinson CD, Cho TJ, Thanh VH, Scallon BJ, Fischer RL, Sims TL, Drews GN, Goldberg RB (1989) Characterization of the glycinin gene family in soybean. The Plant Cell 1, 313–328.

Nishinari K, Kohyama K, Zhang Y, Kitamura K, Sugimoto T, Saio K, Kawamura Y (1991) Rheological study on the effect of the A5-subunit on the gelation characteristics of soybean proteins. Agricultural and Biological Chemistry 55, 351–355.
Rheological study on the effect of the A5-subunit on the gelation characteristics of soybean proteins.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXhs1aksb4%3D&md5=e46279c46d2681fbce5bf9e9f5ee877dCAS |

Obatolu VA (2008) Effect of different coagulants on yield and quality of tofu from soymilk. European Food Research and Technology 226, 467–472.
Effect of different coagulants on yield and quality of tofu from soymilk.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXks1Cmsw%3D%3D&md5=4ab9c1e3516b4211bd1b9d7f8fcc9b71CAS |

Poysa V, Woodrow L, Yu K (2006) Effect of soy protein subunit composition on tofu quality. Food Research International 39, 309–317.
Effect of soy protein subunit composition on tofu quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlajtbjM&md5=05cad984d592283fab0d4477976ef850CAS |

Renkema JMS, Knabben JHM, van Vliet T (2001) Gel formation by beta-conglycinin and glycinin and their mixtures. Food Hydrocolloids 15, 407–414.
Gel formation by beta-conglycinin and glycinin and their mixtures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXosVCksbY%3D&md5=e77f1908eaedb0c83c2240e9b64da1c3CAS |

Saio K (1985) Tofu processing characteristics of Japanese domestic soybeans. Report of the National Food Research Institute [Shokuryo Kenkyusho Kenkyu Hokoku], pp. 128–149.

Saio K, Watanabe T (1978) Differences in functional properties of 7s and 11s soybean proteins. Journal of Texture Studies 9, 135–157.
Differences in functional properties of 7s and 11s soybean proteins.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXmtFeltro%3D&md5=faf9ac98a1aef78486c2672c749dc365CAS |

Saio K, Kamiya M, Watanabe T (1969) Food processing characteristics of soybean-11s and soybean-7s proteins. I. Effect of difference of protein components among soybean varieties on formation of tofu-gel. Agricultural and Biological Chemistry 33, 1301–1308.
Food processing characteristics of soybean-11s and soybean-7s proteins. I. Effect of difference of protein components among soybean varieties on formation of tofu-gel.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3cXit1Ol&md5=1c0f83d29362adde77572ca7b19e72cfCAS |

Schaefer MJ, Love J (1992) Relationships between soybean components and tofu texture. Journal of Food Quality 15, 53–66.
Relationships between soybean components and tofu texture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XkvV2jtbc%3D&md5=bc154a0ee2779ed36d7b2c07d1236a8bCAS |

Shannon JG, Wilcox JR, Probst AH (1972) Estimated gains from selection for protein and yield in F4 generations of 6 soybean populations. Crop Science 12, 824–826.
Estimated gains from selection for protein and yield in F4 generations of 6 soybean populations.Crossref | GoogleScholarGoogle Scholar |

Shen CF, Deman L, Buzzell RI, Deman JM (1991) Yield and quality of tofu as affected by soybean and soymilk characteristics—glucono-delta-lactone coagulant. Journal of Food Science 56, 109–112.
Yield and quality of tofu as affected by soybean and soymilk characteristics—glucono-delta-lactone coagulant.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXktVOhu7s%3D&md5=2d5452428f5b842234d632a54440bf8dCAS |

Stanojevic SP, Barac MB, Pesic MB, Vucelic-Radovic BV (2011) Assessment of soy genotype and processing method on quality of soybean tofu. Journal of Agricultural and Food Chemistry 59, 7368–7376.
Assessment of soy genotype and processing method on quality of soybean tofu.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXntVOktr8%3D&md5=35c6feda005dbe684c92c63af18ce695CAS | 21627319PubMed |

Taira H, Tanaka H, Saito M (1990) Quality of soybean seeds grown in Japan. 20: Differences in sugar contents of soybeans cultured by upland and drained paddy field. Journal of the Japanese Society for Food Science and Technology 37, 602–611.
Quality of soybean seeds grown in Japan. 20: Differences in sugar contents of soybeans cultured by upland and drained paddy field.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXhvVCjsbY%3D&md5=4201c3a74059ac4bef5a9c1a90a26ff0CAS |

Tezuka M, Taira H, Igarashi Y, Yagasaki K, Ono T (2000) Properties of tofus and soy milks prepared from soybeans having different subunits of glycinin. Journal of Agricultural and Food Chemistry 48, 1111–1117.
Properties of tofus and soy milks prepared from soybeans having different subunits of glycinin.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXhvVyrsrw%3D&md5=6577dcf4e2667c5a67d8d65692f09cfbCAS | 10775358PubMed |

Wang CCR, Chang SKC (1995) Physicochemical properties and tofu quality of soybean cultivar Proto. Journal of Agricultural and Food Chemistry 43, 3029–3034.
Physicochemical properties and tofu quality of soybean cultivar Proto.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXpsFWqtbc%3D&md5=7bdcc798cf60f13e2c6927f60d1a2944CAS |

Wang HL, Swain EW, Kwolek WF (1983) Effect of soybean varieties on the yield and quality of tofu. Cereal Chemistry 60, 245–248.

Yang A, James AT (2012) Texture and water holding of silken tofu prepared with two small-scale methods and two coagulants. In ‘Abstracts of the 45th Australian Institute of Food Science and Technology’. Adelaide, S. Aust. p. 47. (Australian Institute of Food Science and Technology: North Ryde, NSW)

Yang A, James AT (2013) Effects of soybean protein composition and processing conditions on silken tofu properties. Journal of the Science of Food and Agriculture 93, 3065–3071.
Effects of soybean protein composition and processing conditions on silken tofu properties.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXmt1Sru7g%3D&md5=752d031ec2c494628d6b8ff9f53c310eCAS | 23512756PubMed |

Yang A, James AT (2014) Influence of globulin subunit composition of soybean proteins on silken tofu quality. 1. Effect of growing location and 11SA4 and 7Sα’ deficiency. Crop & Pasture Science 65, 259–267.
Influence of globulin subunit composition of soybean proteins on silken tofu quality. 1. Effect of growing location and 11SA4 and 7Sα’ deficiency.Crossref | GoogleScholarGoogle Scholar |

Zhang B, Chen P, Florez-Palacios SL, Shi A, Hou A, Ishibashi T (2010) Seed quality attributes of food-grade soybeans from the US and Asia. Euphytica 173, 387–396.
Seed quality attributes of food-grade soybeans from the US and Asia.Crossref | GoogleScholarGoogle Scholar |