Histology of organogenesis and somatic embryogenesis in excised root cultures of an endangered species Tylophora indica (Asclepiadaceae)
Aastha Sahai A , Anwar Shahzad A B and Shiwali Sharma AA Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh-202002 (U.P.), India.
B Corresponding author. Email: ashahzad.bt@amu.ac.in
Australian Journal of Botany 58(3) 198-205 https://doi.org/10.1071/BT09220
Submitted: 22 November 2009 Accepted: 24 February 2010 Published: 5 May 2010
Abstract
This paper reports an efficient regeneration protocol through parallel organogenic and embryogenic pathways from green root segments (GRSs) of Tylophora indica (Burm.f) Merrill. GRSs explants from one year old in vitro cultures were cultured on Murashige and Skoog (MS) medium containing various cytokinins. Five µmol/L of 6-benzyladenine (BA) was most responsive for organogenesis in 1.5 cm long GRSs. Repeated subculture on medium containing both BA (5 µmol/L) and 1-naphthleneacetic acid (NAA) (0.1 µmol/L) promoted multiplication and proliferation of direct shoot buds (46.80 ± 0.96) and callus mediated somatic embryogenesis (18.07 ± 0.33). Germinated embryos isolated from callus were transferred onto maturation medium consisting of half-strength MS medium either devoid of plant growth regulators (PGRs) or with various concentrations of gibberellic acid (GA). Microshoots were excised during subculture and transferred onto root induction medium, thus ensuring a continuous supply of germplasm. Morphogenic variations were noticed in types of roots induced on various auxins. Regenerated plantlets and emblings hardened best on vermiculite with a survival rate of 90% and 70% respectively. However, the emblings were healthier in comparison to the regenerated plants. Histological analysis showed the origin and development of organogenesis.
Acknowledgements
Dr Anwar Shahzad gratefully acknowledges the financial support provided by the Department of Science and Technology (DST), Government of India, New Delhi in the form of SERC Fast Track Scheme Vide no. SR/FT/L-23/2006. Authors are also thankful to the Department of Science and Technology (DST), Government of India, New Delhi for providing research assistance under DST-FIST Program 2005 (Project No. SR/FST/LSI-085/2005).
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