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RESEARCH ARTICLE
Contents Vol 57(11)

Growth regulators restore germination of Orobanche seeds that are conditioned under water stress and suboptimal temperature

W. J. Song A , W. J. Zhou A D , Z. L. Jin A , D. Zhang A , K. Yoneyama B , Y. Takeuchi B and D. M. Joel C
+ Author Affiliations
- Author Affiliations

A Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.

B Weed Science Center, Utsunomiya University, Utsunomiya 321-8505, Japan.

C Department of Phytopathology and Weed Research, ARO, Newe-Ya’ar Research Center, 30095 Israel.

D Corresponding author. Email: wjzhou@zju.edu.cn

Australian Journal of Agricultural Research 57(11) 1195-1201 https://doi.org/10.1071/AR06131
Submitted: 1 May 2006  Accepted: 3 August 2006   Published: 27 October 2006

Abstract

Broomrapes (Orobanche spp.) are serious root parasitic weeds that cause great damage to crop production in many parts of the world. The study focussed on the influence of plant growth regulators on germination of Orobanche seeds conditioned under suboptimal temperature (at 13°C) and under water stress (at –1 and –2 MPa). Three widely distributed species of broomrapes (O. aegyptiaca, O. ramosa, and O. minor) were used in the experiments. Exogenous GA3 (10 mg/L), brassinolide (1 mg/L), and fluridone (10 mg/L) significantly increased the broomrape seed response to the germination stimulant GR24 (10–6 m) even when seeds were first conditioned at a suboptimal temperature and under water stress. The highest germination was obtained when the combined treatments with fluridone and brassinolide, or with GA3 and brassinolide were applied together with the germination stimulant. This indicates that there were additive effects among various plant growth regulators in the regulation of germination response in Orobanche seeds. With the prolongation of conditioning periods under low temperature stress, the restoration capacities of seed germination by a single growth regulator decreased, but the combined treatments of growth regulators retained their positive effects in restoring seed germination.

Additional keywords: broomrape (Orobanche spp.), seed conditioning, plant growth regulators, temperature effect, water potential.


Acknowledgments

The project was supported by the National Natural Science Foundation of China (30170554, 30370852, 30500333), the Special Programme for Doctoral Discipline of the China Ministry of Education (20040335011), the 111 Project from the China Ministry of Education and the State Administration of Foreign Experts Affairs (B06014), and ‘Grant-in-Aid for Scientific Research’ from the Japan Society for the Promotion of Science (15380079).


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